CLPP Antibody

Western Blot: CLPP Antibody [NB100-1189] - Analysis in Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Immunohistochemistry-Paraffin: CLPP Antibody [NB100-1189] - IHC staining of CLPP in paraffin embedded Human Liver using this antibody at 2.5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: CLPP Antibody [NB100-1189] - Analysis of CLPP in human muscle lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 1 ug/ml. Primary antibody incubated for 1 hour. Detected using chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CLPP Antibody Summary

• Immunogen: Peptide with sequence C-EAAPAAEPVPAST corresponding to C-Terminus according to NP_006003.1.
• Localization: Mitochondrial
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLPP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in human heart (calculated MW of 30.2 kDa band according to NP_006003.1). IHC-P: Human liver shows cytoplasmic staining consistent with mitochondrai in hepatocytes.
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  CLPP Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CLPP Antibody

• ATP-dependent protease ClpAP, proteolytic subunit, human
• ClpP (caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli)homolog
• ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E.coli)
• ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E.coli)
• ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog
• EC 3.4.21.92
• Endopeptidase Clp
• putative ATP-dependent Clp protease proteolytic subunit, mitochondrial

Background

CLPP belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLPP Antibody (NB100-1189)(4)

Publications using NB100-1189

• Telionis A, Lahmers K, Todd M et al. Distribution of Theileria orientalis in Virginia Market Cattle, 2018–2020 Pathogens 2022-11-15 [PMID: 36422604]
• Hainley LE, Hughson MS, Narendran A et al. Chk1 and the Host Cell DNA Damage Response as a Potential Antiviral Target in BK Polyomavirus Infection Viruses 2021-07-13 [PMID: 34372559]
• Sluyter JD, Camargo CA, Waayer D et al. Effect of Monthly, High-Dose, Long-Term Vitamin D on Lung Function: A Randomized Controlled Trial Nutrients 2017-12-13 [PMID: 29236049]
• Bross P, Andresen BS, Knudsen I et al. Human ClpP protease: cDNA sequence, tissue-specific expression and chromosomal assignment of the gene. FEBS Lett 1995-12-18 [PMID: 8543061]

Bioinformatics

• Gene Symbol: CLPP
• Entrez:
  • 8192(Human)
• Uniprot:
  • NP_006003.(Human)