CLPP Antibodies
We offer CLPP Antibodies for use in common research applications: Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each CLPP Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CLPP Antibodies can be used in a variety of model species: Canine, Human, Mouse, Primate, Rat. Use the list below to choose the CLPP Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CLPP polyclonal antibodies and browse our CLPP monoclonal antibody catalog.
Alternate Names for CLPP Antibodies
anti-CLPP antibody, anti-ATP-dependent protease ClpAP, proteolytic subunit, human antibody, anti-ClpP (caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli)homolog antibody, anti-ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E.coli) antibody, anti-ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E.coli) antibody, anti-ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog antibody, anti-EC 3.4.21.92 antibody, anti-Endopeptidase Clp antibody, anti-putative ATP-dependent Clp protease proteolytic subunit, mitochondrial antibody
12 Results for "clpp" in Primary Antibodies
![Western Blot: CLPP Antibody [NBP1-89557] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CLPP antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP1-89557-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CLPP Antibody [NBP1-89557] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CLPP antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CLPP Antibody [NBP1-89557] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89557-img0010.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0015.jpg)
![Western Blot: CLPP Antibody [NBP1-89557] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP1-89557-img0007.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0012.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0013.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0014.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (OTI1F6) [NBP2-03323] - CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLPP mouse monoclonal antibody.CLPP Antibody (OTI1F6)](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0005.jpg "Immunohistochemistry-Paraffin: CLPP Antibody (OTI1F6) [NBP2-03323] - CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLPP mouse monoclonal antibody.CLPP Antibody (OTI1F6)")
![Western Blot: CLPP Antibody (1F6) [NBP2-03323] Analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Western-Blot-NBP2-03323-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CLPP Antibody (1F6) [NBP2-03323] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunocytochemistry-Immunofluorescence-NBP2-03323-img0010.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Human liver tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0009.jpg)
![Flow Cytometry: CLPP Antibody (1F6) [NBP2-03323] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CLPP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Flow-Cytometry-NBP2-03323-img0001.jpg)
![Western Blot: CLPP Antibody (1F6) [NBP2-03323] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Western-Blot-NBP2-03323-img0002.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0003.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0004.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0006.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0007.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Human Kidney tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0008.jpg)
![Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Western-Blot-NBP2-71363-img0002.jpg "Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.")
![Immunocytochemistry/Immunofluorescence: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71363-img0004.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Human Kidney tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0010.jpg)
![Flow Cytometry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CLPP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71363-img0003.jpg)
![Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Western-Blot-NBP2-71363-img0001.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0005.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0006.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0007.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0008.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0009.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Human liver tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0011.jpg)
![Western Blot: CLPP Antibody [NB100-1189] - Analysis in Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NB100-1189-img0006.jpg "Western Blot: CLPP Antibody [NB100-1189] - Analysis in Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence")
![Immunohistochemistry-Paraffin: CLPP Antibody [NB100-1189] - IHC staining of CLPP in paraffin embedded Human Liver using this antibody at 2.5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NB100-1189-img0004.jpg)
![Western Blot: CLPP Antibody [NB100-1189] - Analysis of CLPP in human muscle lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 1 ug/ml. Primary antibody incubated for 1 hour. Detected using chemiluminescence.](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NB100-1189-img0005.jpg)
![Western Blot: CLPP Antibody (1W6O4) [NBP3-16156] - Western blot analysis of extracts of various cell lines, using CLPP Rabbit mAb (NBP3-16156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CLPP-Antibody-1W6O4-Western-Blot-NBP3-16156-img0004.jpg "Western Blot: CLPP Antibody (1W6O4) [NBP3-16156] - Western blot analysis of extracts of various cell lines, using CLPP Rabbit mAb (NBP3-16156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: CLPP Antibody (1W6O4) [NBP3-16156] - Immunofluorescence analysis of NIH-3T3 cells using CLPP Rabbit mAb (NBP3-16156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CLPP-Antibody-1W6O4-Immunocytochemistry-Immunofluorescence-NBP3-16156-img0003.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1W6O4) [NBP3-16156] - Immunohistochemistry of paraffin-embedded human colon using CLPP Rabbit mAb (NBP3-16156) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLPP-Antibody-1W6O4-Immunohistochemistry-Paraffin-NBP3-16156-img0002.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1W6O4) [NBP3-16156] - Immunohistochemistry of paraffin-embedded rat kidney using CLPP Rabbit mAb (NBP3-16156) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CLPP-Antibody-1W6O4-Immunohistochemistry-Paraffin-NBP3-16156-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody (1W6O4) [NBP3-16156] - Immunofluorescence analysis of NIH/3T3 cells using CLPP Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16156_rabbit-clpp-mab-1w6o4-5220251242582.jpg)
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody (1W6O4) [CLPP] - Immunofluorescence analysis of NIH/3T3 cells using CLPP Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16156_rabbit-clpp-mab-1w6o4-5220251734525.jpg)
![Western Blot: CLPP Antibody (3E2) [H00008192-M01] - CLPP monoclonal antibody (M01), clone 3E2. Analysis of CLPP expression in PC-12.](http://images.novusbio.com/fullsize/CLPP-Antibody-3E2-Western-Blot-H00008192-M01-img0007.jpg "Western Blot: CLPP Antibody (3E2) [H00008192-M01] - CLPP monoclonal antibody (M01), clone 3E2. Analysis of CLPP expression in PC-12.")
![Immunohistochemistry-Paraffin: CLPP Antibody (3E2) [H00008192-M01] - Analysis of monoclonal antibody to CLPP on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/CLPP-Antibody-3E2-Immunohistochemistry-Paraffin-H00008192-M01-img0005.jpg)
![Western Blot: CLPP Antibody (3E2) [H00008192-M01] - CLPP monoclonal antibody (M01), clone 3E2 Analysis of CLPP expression in A-431.](http://images.novusbio.com/fullsize/CLPP-Antibody-3E2-Western-Blot-H00008192-M01-img0008.jpg)
![Sandwich ELISA: CLPP Antibody (3E2) [H00008192-M01] - Detection limit for recombinant GST tagged CLPP is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-3E2-Sandwich-ELISA-H00008192-M01-img0006.jpg)
![Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-278202416285288.jpg "Western Blot: CLPP Antibody (JE40-00) [NBP3-32195] - Western blot analysis of CLPP on different lysates with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SW620 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (39 ug/Lane) Predicted band size: 30 kDa Observed band size: 25 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CLPP Antibody (JE40-00) [NBP3-32195] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CLPP antibody (NBP3-32195) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32195) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29820241492667.jpg)
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody (JE40-00) [NBP3-32195] - Immunocytochemistry analysis of HeLa cells labeling CLPP with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLPP antibody (NBP3-32195) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202410334080.jpg)
![Flow Cytometry: CLPP Antibody (JE40-00) [NBP3-32195] - Flow cytometric analysis of HeLa cells labeling CLPP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32195, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32195_rabbit-clpp-mab-je40-00-29202416372131.jpg)
![Western Blot: CLPP Antibody [NBP1-31011] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody diluted by 1:500.](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP1-31011-img0004.jpg "Western Blot: CLPP Antibody [NBP1-31011] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody diluted by 1:500.")
![Immunocytochemistry/Immunofluorescence: CLPP Antibody [NBP1-31011] - Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: CLPP protein stained by CLPP antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31011-img0005.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-31011] - Paraffin-embedded H520 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-31011-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CLPP Antibody [NBP1-31011] - Wild-type (WT) and CLPP knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31011_rabbit-polyclonal-clpp-antibody-1610202419414599.jpg)
![Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP3-06239-img0003.jpg "Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa")
![Immunocytochemistry/Immunofluorescence: CLPP Antibody [NBP3-06239] - Immunofluorescence staining of CLPP in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CLPP polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06239-img0001.jpg)
![Immunoprecipitation: CLPP Antibody [NBP3-06239] -](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunoprecipitation-NBP3-06239-img0002.jpg)
![Western Blot: CLPP Antibody [NBP2-15919] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP2-15919-img0001.jpg "Western Blot: CLPP Antibody [NBP2-15919] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP2-15919] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP2-15919-img0002.jpg)
![Western Blot: CLPP Antibody (S02-1E5) [NBP3-15014] - Western blot detection of CLPP in K562, Rat Brain, 3T3, Hela cell lysates using CLPP Rabbit mAb (1:1000 diluted). Predicted band size: 30kDa. Observed band size: 30kDa.](http://images.novusbio.com/fullsize/CLPP-Antibody-S02-1E5-Western-Blot-NBP3-15014-img0001.jpg "Western Blot: CLPP Antibody (S02-1E5) [NBP3-15014] - Western blot detection of CLPP in K562, Rat Brain, 3T3, Hela cell lysates using CLPP Rabbit mAb (1:1000 diluted). Predicted band size: 30kDa. Observed band size: 30kDa.")