CLOCK Antibody (NBP1-30326)

Images

 
Western Blot: CLOCK Antibody [NBP1-30326] - Analysis of CLOCK in Sol8 (mouse embryo fibroblast) cell extracts using NBP1-30326.
Immunohistochemistry: CLOCK Antibody [NBP1-30326] - CLOCK antibody was tested in mouse brain using DAB with hematoxylin counterstain.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-30326
    • Availability
      Product Discontinued

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CLOCK Antibody Summary

Immunogen
Synthetic peptide made to a C-terminal portion of mouse CLOCK (within residues 805-855). [Swiss-Prot# O08785]
Localization
Cytoplasmic and Nuclear. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus.
Predicted Species
Rat (94%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
CLOCK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:100
Application Notes
This CLOCK antibody is useful for Western blot analysis where a band can be seen at 95 kDa. This antibody is not recommended for Immunocytochemistry/Immunofluorescence.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Lysate (NB820-59177)

Reactivity Notes

Human and mouse. Immunogen has 94% identity with rat. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CLOCK Antibody

  • BHLHE8
  • circadian locomoter output cycles protein kaput
  • Class E basic helix-loop-helix protein 8
  • clock (mouse) homolog
  • clock homolog (mouse)
  • CLOCK
  • EC 2.3.1.48
  • hCLOCK
  • KAT13D
  • KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Background

Different physiological as well as behavioral processes such as sleep-wake cycles, thermoregulation, hormonal secretions, blood pressure and metabolism exhibits 24-hour rhythmicity which is generated through a molecular machinery involving transcriptional-translational feedback loop in the suprachiasmatic nucleus (SCN) of hypothalamus and many peripheral tissues. Mammalian circadian system contains a set of core CLOCK genes that encode proteins such as CLOCK (circadian locomotor output kaput), BMAL1 (brain and muscle ARNLT1), PER (period), CRY (cryptochrome), REV-ERB alpha, and ROR alpha. CLOCK was the first gene identified in its family and CLOCK protein binds DNA for activating transcription after dimerization with BMAL1 by driving the rhythmic transcription of other CLOCK and circadian clock-controlled genes through an E-box (5'-CACGTG-3'). It activates transcription of PER1/PER2 which is inhibited in a feedback loop by PER and CRY proteins. With its intrinsic histone acetyltransferase activity, CLOCK acetylates histones H3, histone H4, and ARNTL (non-histone substrate), and has also been shown to plays a role in DNA damage response/DDR signaling during the S phase of cell cycle. Because this endogenous timekeeper interacts with countless biological systems, CLOCK dysfunctions may lead to the pathogenesis of several diseases including sleep disorders, metabolic syndrome, cardiovascular and inflammatory disease, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CLOCK
Entrez
Uniprot