CLIP170 Antibody

Images

 
Western Blot: CLIP170 Antibody [NBP1-36749] - Analysis of CLIP170 in Human Breast Cancer lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-36749] - (2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CLIP170 Antibody Summary

Immunogen
Peptide with sequence C-PHSTHHGSRGEERP corresponding to C-Terminus according to NP_937883.1.
Epitope
C-Terminus
Specificity
This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CLIP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 160 kDa band observed in human breast cancer lysates (calculated MW of 161 kDa band according to NP_002947.1. IHC-P: Human thyroid gland shows structured cytoplasm staining in active epithelial cells.
Publications
Read Publication using NBP1-36749.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CLIP170 Antibody

  • CAP-GLY domain containing linker protein 1
  • CLIP
  • CLIP170
  • CLIP-170Reed-Sternberg intermediate filament-associated protein
  • CYLN1MGC131604
  • cytoplasmic linker 1
  • Cytoplasmic linker protein 1
  • Cytoplasmic linker protein 170 alpha-2
  • Reed-Steinberg cell-expressed intermediate filament-associated protein
  • restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
  • Restin
  • RSNCAP-Gly domain-containing linker protein 1

Background

Cytoplasmic linker protein of 170 kDa (CLIP-170) is the original member of a group of microtuble binding proteins designated as plus-end-binding proteins(+TIPs). CLIP-170 binds to the growing plus ends of microtubules and acts as a linker between the dynamic microtubule ends and organelle membranes. The protein acts as an anticatastrophic factor, promoting microtubule rescue near the cell periphery. Fluorescently labeled CLIP-170 can be visualized as a comet like streak around the growing ends of microtubules. CLIP-170 colocalizes with dynactin and dynein at microtubule ends and also at the kinetochore. Restin, first identified as a marker for Hodgkin and Reed-Sternberg (HRS) cells, is a 160 kDa splice variant of the gene that includes a 35 amino acid stretch not present in CLIP-170. CLIP-170/restin is highly expressed in HRS cells, monocyte-derived dendritic cells, IL-4 + CD40L activated B cells, and in Ki-1 lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CLIP170 Antibody (NBP1-36749)(1)

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Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

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Bioinformatics

Gene Symbol CLIP1
Entrez
Uniprot