CLIC4 Antibody

Western Blot: CLIC4 Antibody [NBP1-00172] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP1-00172] - (5ug/ml) staining of min6 cells transiently expressing mouse Clic4. Nuclear counter staining by Hoechst. Data kindly provided by Dr. Virginie Nepote, Lausanne University, Switzerland.

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-00172] - (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CLIC4 Antibody Summary

• Description: Novus Biologicals Goat CLIC4 Antibody (NBP1-00172) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-CLIC4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence NGLKEEDKEPLIE-C corresponding to N-Terminus according to NP_039234.1.
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLIC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA detection limit 1:64000
  • Western Blot 0.1-0.3 ug/ml
• Application Notes: IF: Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells. WB: Approx. 30 kDa band observed in human kidney and human placenta lysates is reported in scientific literature (PMID: 10070163).
• Control:

  CLIC4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CLIC4 Antibody

• chloride intracellular channel 4 like
• chloride intracellular channel 4
• chloride intracellular channel protein 4
• CLIC4L
• DKFZp566G223
• FLJ38640
• H1
• huH1
• Intracellular chloride ion channel protein p64H1
• MTCLIC
• P64H1

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLIC4 Antibody (NBP1-00172)(2)

Publications using NBP1-00172

• James J, Dekan A, Niihori M et al. Novel Populations of Lung Capillary Endothelial Cells and Their Functional Significance Research square 2023-05-03 [PMID: 37205391] (IHC-P, FLOW, Rat)
• Suh KS, Crutchley JM, Koochek A et al. Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers. Clin Cancer Res 2007-01-01 [PMID: 17200346]

Bioinformatics

• Gene Symbol: CLIC4
• Entrez:
  • 25932(Human)
  • 29876(Mouse)
  • 83718(Rat)
• Uniprot:
  • NP_039234.1(Human)