CLIC4 Overexpression Lysate (Native)

Images

 
Western Blot: CLIC4 Overexpression Lysate (Adult Normal) [NBL1-09269] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CLIC4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CLIC4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201893 and based on accession number NM_013943. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chloride intracellular channel 4 (CLIC4), nuclear gene encoding mitochondrial protein, mRNA.
Gene
CLIC4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CLIC4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CLIC4 Overexpression Lysate (Native)

  • chloride intracellular channel 4 like
  • chloride intracellular channel 4
  • chloride intracellular channel protein 4
  • CLIC4L
  • DKFZp566G223
  • FLJ38640
  • H1
  • huH1
  • Intracellular chloride ion channel protein p64H1
  • MTCLIC
  • P64H1

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CLIC4 Lysate (NBL1-09269) (0)

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Bioinformatics

Gene Symbol CLIC4