CLEC9a Antibody (683409) [Allophycocyanin]

HEK293 human embryonic kidney cell line transfected with human CLEC9a and eGFP was stained with either (A) Mouse Anti-Human CLEC9a APC-conjugated Monoclonal Antibody (Catalog # FAB6049A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood cells gated on CD3-CD141+cells were stained with Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (Catalog # FAB4869P) and either (A) Mouse Anti-Human CLEC9a APC-conjugated Monoclonal Antibody (Catalog # FAB6049A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 683409
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CLEC9a Antibody (683409) [Allophycocyanin] Summary

• Additional Information: Clone 683409 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CLEC9a
  Lys57-Val241
  Accession # Q6UXN8
• Specificity: Detects human CLEC9a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC1, 2, 2A, 3B, 10A, 12B, 14A, rhCD302/CLEC13a, rhMICL, or recombinant mouse CLEC9a is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC9A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC9a Antibody (683409) [Allophycocyanin]

• CD370
• CLEC9a
• C-type lectin domain family 9 member A
• C-type lectin domain family 9, member A
• DNGR1
• HEEE9341
• UNQ9341

Background

CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C‑type lectin superfamily (1, 2). Mature human CLEC9a consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (3‑5). Within the ECD, human CLEC9a shares 57% aa sequence identity with mouse and rat CLEC9a. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits (3, 5). Human CLEC9a expression is restricted to a subpopulation of BDCA-3⁺ conventional dendritic cells (cDC) and CD16^- monocytes (3‑5). BDCA-3⁺ cDC are analagous to mouse CD8⁺ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells (4, 5). CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (3‑5). In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell (7). The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris (7). CLEC9a signaling triggers activation of the tyrosine kinase Syk (3, 7).

1. Huysamen, C. and G.D. Brown (2009) FEMS Microbiol. Lett. 290:121.
2. Geijtenbeek, T.B.H. et al. (2004) Annu. Rev. Immunol. 22:33.
3. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693.
4. Caminschi, I. et al. (2008) Blood 112:3264.
5. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098.
6. Dudziak, D. et al. (2007) Science 315:107.
7. Sancho, D. et al. (2009) Nature 458:899.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC9a Antibody (FAB6049A)(2)

Publications using FAB6049A

• Balan S, Ollion V, Colletti N, Chelbi R, Montanana-Sanchis F, Liu H, Vu Manh T, Sanchez C, Savoret J, Perrot I, Doffin A, Fossum E, Bechlian D, Chabannon C, Bogen B, Asselin-Paturel C, Shaw M, Soos T, Caux C, Valladeau-Guilemond J, Dalod M Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells. J Immunol, 2014-07-09;193(4):1622-35. 2014-07-09 [PMID: 25009205] (Flow Cytometry, Human)
• Dutertre C, Jourdain J, Rancez M, Amraoui S, Fossum E, Bogen B, Sanchez C, Couedel-Courteille A, Richard Y, Dalod M, Feuillet V, Cheynier R, Hosmalin A TLR3-responsive, XCR1+, CD141(BDCA-3)+/CD8alpha+-equivalent dendritic cells uncovered in healthy and simian immunodeficiency virus-infected rhesus macaques. J Immunol, 2014-04-16;192(10):4697-708. 2014-04-16 [PMID: 24740505] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))

Bioinformatics

• Gene Symbol: CLEC9A
• Uniprot:
  • Q6UXN8()