CLEC-2/CLEC-1B Antibody (219150) [Unconjugated]

Western blot shows lysates of human platelets. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human CLEC-2/CLEC1B Monoclonal Antibody (Catalog # MAB1718) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC-2/CLEC1B at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

• Reactivity: Hu
• Applications: WB
• Clone: 219150
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CLEC-2/CLEC-1B Antibody (219150) [Unconjugated] Summary

• Immunogen: NS0-derived recombinant human CLEC-2 extracellular domain
  Gln58-Pro229
  Accession # AAF36777
• Specificity: Detects human CLEC-2/CLEC1B in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human
  CLEC-1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC-2/CLEC-1B Antibody (219150) [Unconjugated]

• 1810061I13Rik
• CLEC1B
• CLEC2
• CLEC-2
• CLEC2B
• CLEC2PRO1384
• C-type lectin domain family 1 member B
• C-type lectin domain family 1, member B
• C-type lectin-like receptor 2
• C-type lectin-like receptor-2
• QDED721

Background

C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the
C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca²⁺-dependent carbohydrate binding, making it a
non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the
platelet-aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).

1. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
2. Christou, C.M. et al. (2008) Biochem. J. 411:133.
3. Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
4. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
5. Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
6. Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
7. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
8. Chaipan, C. et al. (2006) J. Virol. 80:8951.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC-2/CLEC-1B Antibody (MAB1718)(4)

Publications using MAB1718

• Yohei Takahashi, Hidenori Wake, Masakiyo Sakaguchi, Yukinori Yoshii, Kiyoshi Teshigawara, Dengli Wang et al. Histidine-Rich Glycoprotein Stimulates Human Neutrophil Phagocytosis and Prolongs Survival through CLEC1A The Journal of Immunology 2021-02-15 [PMID: 33452125]
• R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study Cell Death Dis, 2017-08-24;8(8):e3017. 2017-08-24 [PMID: 28837142] (Western Blot, Human)
• Chang C, Chung C, Hsu C, Peng H, Huang T Inhibitory effects of polypeptides derived from a snake venom C-type lectin, aggretin, on tumor cell-induced platelet aggregation. J Thromb Haemost, 2014-04-01;12(4):540-9. 2014-04-01 [PMID: 24479713] (Western Blot, Human)
• Chang CH, Chung CH, Hsu CC, Huang TY, Huang TF A novel mechanism of cytokine release in phagocytes induced by aggretin, a snake venom C-type lectin protein, through CLEC-2 ligation. J. Thromb. Haemost., 2010-11-01;8(11):2563-70. 2010-11-01 [PMID: 20738764] (Flow Cytometry, Western Blot, Human)

Bioinformatics

• Gene Symbol: CLEC1B
• Uniprot:
  • AAF36777()