CLEC-1 Antibody

Western Blot: CLEC-1 Antibody [NBP2-27096] - Analysis of CLEC1 using CLEC1 antibody. Human lung tissue lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of CLEC1 antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: CLEC-1 Antibody [NBP2-27096] - Human lung tissue probed with CLEC1 antibody at 5 ug/ml.

• Reactivity: Hu, Mu, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CLEC-1 Antibody Summary

• Immunogen: A portion of amino acids 50-100 from human CLEC1 was used as the immunogen for this antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLEC1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 2-4ug/ml~

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:10.1167/iovs.62.6.28). Orangutan, chimpanzee, and white-cheeked gibbon (100%), New world monkey, crab-eating macaque, and rhesus monkey (89%), Porcine (78%), Canine, african bush elephant, and rabbit (72%), panda, cattle, chinese hamster, and horse (67%), Rat (61%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for CLEC-1 Antibody

• CLEC1
• CLEC-1
• CLEC1A
• CLEC1C-type lectin domain family 1 member A
• C-type lectin domain family 1, member A
• C-type lectin-like receptor 1
• C-type lectin-like receptor-1
• MGC34328

Background

CLEC1 (CLEC-1) with a molecular weight of ~32kDa, is part of the superfamily of C-type lectin proteins (CLRs) and also part of the Dectin-1 cluster subgroup which includes LOX-1, , MICL, CLEC12B, and besides CLEC1 (CLEC-1) and Dectin-1 (1, 2). The Dectin-1 cluster especially has been shown to be instrumental in regulating immunity and immune homeostasis. C-type lectin proteins, including CLEC1, have been characterized as Pattern Recognition Receptors (PRRs), binding both exogenous microbial ligands and Pathogen Associate Molecular Pattern molecules (PAMPs) as well as endogenous Damage Associated Molecular Pattern Molecules (DAMPs) such as self-antigens or altered self-antigens (1, 3, 4). CLEC1 is expressed on myeloid cells and Dendritic Cells and more moderately expressed on endothelial cells in tolerated allografts in a rat model system (1). In said model, CLEC1 expression is down-modulated in DCs, with concomitant increases in T cells and decreases in + Treg pools. Expression of CLEC1 is down regulated by pro-inflammatory stimuli and up-regulated by IL-10 and TGF in normal rat cells (1). Taken together, CLEC1 as other CLRs appears to play an important functional role in modulation of adaptive immune responses through stimulation of cytokine cascades and subsequent activation of polarized T cell responses. CLEC1 specific antibody is a useful reagent for examining the potential of CLEC1 as a marker or therapeutic target for regulation of immune responses in inflammation or cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC-1 Antibody (NBP2-27096)(1)

Publication using NBP2-27096

• Zhu G, Lyu L, Yang H et al. CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis Investigative ophthalmology & visual science 2021-05-03 [PMID: 34043748] (WB, IF/IHC, Human, Mouse)

Bioinformatics

• Gene Symbol: CLEC1A
• Uniprot:
  • Q8NC01()