CLC Antibody - BSA Free

Western Blot: NR6/CLC Antibody [NBP2-19580] - A. 30 ug 293T whole cell lysate/extractB. 30 ug whole cell lysate/extract of human CLCF1-transfected 293T cells 12% SDS-PAGE NNT-1/BSF-3 antibody dilution: 1:5000.

Immunocytochemistry/Immunofluorescence: NR6/CLC Antibody [NBP2-19580] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: NR6/CLC Antibody [NBP2-19580] - Human colon carcinoma, using NNT-1/BSF-3 antibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CLC Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CLC Antibody - BSA Free (NBP2-19580) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CLC. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLCF1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CLC Antibody - BSA Free

• B-cell stimulating factor 3
• B-cell stimulatory factor 3
• BSF-3
• BSF3BSF-3
• cardiotrophin-like cytokine factor 1
• CISS2
• CLC
• CLCF1
• CLCNNT-1
• CRLF1 associated cytokine-like factor 1
• neurotrophin-1/B-cell stimulating factor-3
• NNT-1
• NNT1B-cell-stimulating factor 3
• Novel neurotrophin-1
• NR6

Background

CLCF1 belongs to the interleukin-6 (IL6; MIM 147620) family of cytokines, which are involved in cell signaling through phosphorylation of gp130 (IL6ST; MIM 600694). IL6 family members share similarity in gene structure and have a 4-helix bundle in their protein structure.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CLCF1
• Uniprot:
  • Q9UBD9()