Claudin-7 Antibody

Western Blot: Claudin-7 Antibody [NBP2-78833] - Detection of human CLDN7 by western blot. Samples: Whole cell lysate (15 ug) from MCF-7, LNCaP, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLDN7 antibody NBP2-78833 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.

Immunoprecipitation: Claudin-7 Antibody [NBP2-78833] - Detection of human CLDN7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CLDN7 antibody NBP2-78833 used for IP at 40 ul per reaction. CLDN7 was also immunoprecipitated by another rabbit anti-CLDN7 antibody. For blotting immunoprecipitated CLDN7, NBP2-78833 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

Product Discontinued

Claudin-7 Antibody Summary

• Immunogen: The epitope recognized by A305-892A-M maps to a region between residue 161 and 211 of human Claudin-7 using the numbering given in entry O95471.4 (GeneID 1366).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLDN7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 20-40 ul/mg lysate
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Tris-buffered Saline, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.04 mg/ml
• Purity: Affinity purified

Alternate Names for Claudin-7 Antibody

• CEPTRL2
• claudin 7
• claudin-1
• Claudin7
• Claudin-7
• CLDN7
• CLDN-7
• clostridium perfringens enterotoxin receptor-like 2
• CPETRL2

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. mRNA studies indicate that claudin-7 is specifically expressed in mouse lung and kidney, but not in heart, brain, spleen, liver, skeletal muscle or testis. The gene encoding human claudin-7 maps to chromosome 17p13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLDN7