Claudin-7 Antibody

Immunohistochemistry-Paraffin: Claudin-7 Antibody [NBP1-02486] - Staining of human kidney tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Product Discontinued

Claudin-7 Antibody Summary

• Immunogen: Synthetic peptide from human CLDN7 / Claudin-7.
• Specificity: Amino acids 107-120 of human Claudin-7
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLDN7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
• Application Notes: IHC: NBP1-02486 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Claudin-7 Antibody

• CEPTRL2
• claudin 7
• claudin-1
• Claudin7
• Claudin-7
• CLDN7
• CLDN-7
• clostridium perfringens enterotoxin receptor-like 2
• CPETRL2

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. mRNA studies indicate that claudin-7 is specifically expressed in mouse lung and kidney, but not in heart, brain, spleen, liver, skeletal muscle or testis. The gene encoding human claudin-7 maps to chromosome 17p13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLDN7
• Entrez:
  • 1366(Human)
• Uniprot:
  • O95471(Human)