Claudin-7 Antibody

Product Discontinued

Claudin-7 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 171-220 of Human Claudin-7.
• Specificity: Claudin 7 (P202) detects endogenous levels of Claudin 7 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLDN7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Claudin 7 (P202) pAb in extracts from 293 K562 cells.
  Immunohistochemistry (IHC) analyzes of Claudin7 (P202) pAb in paraffin-embedded human lung carcinoma tissue. Use in Immunofluoresence was reported in the scientific literature (PMID: 21717359).
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Claudin-7 Overexpression Lysate

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24016781).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Claudin-7 Antibody

• CEPTRL2
• claudin 7
• claudin-1
• Claudin7
• Claudin-7
• CLDN7
• CLDN-7
• clostridium perfringens enterotoxin receptor-like 2
• CPETRL2

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. mRNA studies indicate that claudin-7 is specifically expressed in mouse lung and kidney, but not in heart, brain, spleen, liver, skeletal muscle or testis. The gene encoding human claudin-7 maps to chromosome 17p13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-7 Antibody (NB100-91714)(2)

Publications using NB100-91714

• Hill Steven M, Nesser Nicole K, Johnson-Camacho Katie et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Systems 2017-01-01 [PMID: 28017544] (WB, Mouse)
• Roussel AJ, Knol AC, Bourdeau PJ, Bruet V. Optimization of an Immunohistochemical Method to Assess Distribution of Tight Junction Proteins in Canine Epidermis and Adnexae. J Comp Pathol. 2013-09-06 [PMID: 24016781] (IHC-P, Canine)

FAQs for Claudin-7 Antibody (NB100-91714). (Showing 1 - 1 of 1 FAQs).

1. Is heat mediated antigen retrieval using citrate buffer pH6 required for FFPE sections?
   • A heat mediated antigen retrieval step is not always necessary, but is one that we would recommend for your IHC staining because during fixation the epitope can become masked and undetectable by the antibody. This step is useful in increasing the positive staining that you should see in your samples.

Bioinformatics

• Gene Symbol: CLDN7
• Entrez:
  • 1366(Human)
  • 65132(Rat)
• Uniprot:
  • O95471(Human)