Human Claudin-4 ELISA Kit (Colorimetric)

ELISA: Human Claudin-4 ELISA Kit (Colorimetric) [NBP2-75330] - Samples were spiked with high concentrations of Human Claudin-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Claudin-4 ELISA Kit (Colorimetric) [NBP2-75330] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human Claudin-4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CLDN4 in samples. No significant cross-reactivity or interference between Human CLDN4 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.84%
• Intra-Assay: CV% < 4.68%
• Spike Recovery: 86-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CLDN4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Claudin-4 ELISA Kit (Colorimetric)

• claudin 4
• Claudin4
• Claudin-4
• CLDN4
• Clostridium perfringens enterotoxin receptor 1
• Clostridium perfringens enterotoxin receptor
• CPE-R
• CPE-RCPETR
• CPE-receptor
• CPETR1
• hCPE-R
• WBSCR8
• WBSCR8CPERCPETR1claudin-4
• Williams-Beuren syndrome chromosomal region 8 protein

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CLDN4