Claudin-4 Antibody [Alexa Fluor™ Plus 594]

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 594

Claudin-4 Antibody [Alexa Fluor™ Plus 594] Summary

• Immunogen: Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human Claudin-4. The immunogen is located within the last 50 amino acids of Claudin-4. Amino Acid Sequence: PPRTDKPYSAKYSAAR
• Specificity: At least four isoforms of Claudin-4 are known to exist; Claudin-4 antibody will detect all four isoforms. Human Claudin-4 has one isoform (209aa, 22kD). Mouse Claudin-4 has one isoform (210aa, 22kD) and Rat Claudin-4 also has one isoform (210aa, 22kD). NBP2-41187 can detect human, mouse and rat.
• Predicted Species: Mouse (94%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLDN4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Claudin-4 Antibody [Alexa Fluor™ Plus 594]

• claudin 4
• Claudin4
• Claudin-4
• CLDN4
• Clostridium perfringens enterotoxin receptor 1
• Clostridium perfringens enterotoxin receptor
• CPE-R
• CPE-RCPETR
• CPE-receptor
• CPETR1
• hCPE-R
• WBSCR8
• WBSCR8CPERCPETR1claudin-4
• Williams-Beuren syndrome chromosomal region 8 protein

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLDN4