Human Claudin-1 ELISA Kit (Colorimetric)

ELISA: Human Claudin-1 ELISA Kit (Colorimetric) [NBP2-75324] - Samples were spiked with high concentrations of Human Claudin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Claudin-1 ELISA Kit (Colorimetric) [NBP2-75324] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human Claudin-1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CLDN1 in samples. No significant cross-reactivity or interference between Human CLDN1 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.53%
• Intra-Assay: CV% < 4.52%
• Spike Recovery: 88-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CLDN1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Claudin-1 ELISA Kit (Colorimetric)

• claudin 1
• Claudin1
• Claudin-1
• CLD1
• CLDN1
• ILVASCSenescence-associated epithelial membrane protein
• SEMP1
• SEMP1senescence-associated epithelial membrane protein 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CLDN1