Claudin-1 Antibody [Alexa Fluor™ Plus 555]

• Reactivity: Hu, Mu, Rt, Po, Ca, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 555

Claudin-1 Antibody [Alexa Fluor™ Plus 555] Summary

• Immunogen: Antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human Claudin-1. The immunogen is located within the last 50 amino acids of Claudin-1. Amino Acid Sequence: PRKTTSYPTPRPYPKPAPS
• Specificity: Human Claudin-1 has one isoform (211aa, 23kD). Mouse Claudin-1 has one isoform (211aa, 23kD) and Rat Claudin-1 also has one isoform (211aa, 23kD). NBP1-77036 can detect human, mouse and rat.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLDN1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity per customer review. Mouse reactivity reported in (PMID: 33392105), Canine reactivity reported in (PMID: 32419902).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Claudin-1 Antibody [Alexa Fluor™ Plus 555]

• claudin 1
• Claudin1
• Claudin-1
• CLD1
• CLDN1
• ILVASCSenescence-associated epithelial membrane protein
• SEMP1
• SEMP1senescence-associated epithelial membrane protein 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLDN1