CLASP1 Antibody (KT66) - C-terminus - BSA Free

Western Blot: CLASP1 Antibody (KT66) [NBP3-23219] - Western blot of CLASP1 in mouse brain (lane 1) and rat PC12 cells (lane 2). The blots were probed with rat monoclonal NBP3-23219 anti-CLASP1 (C-terminus) at a dilution of 1:500. Then detected using donkey anti-Rat IgG:HRP.

• Reactivity: Hu, Mu, Rt, Ch, Xp
• Applications: WB, ICC/IF
• Clone: KT66
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free

CLASP1 Antibody (KT66) - C-terminus - BSA Free Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Clone KT66 was generated from a GST fusion protein containing the C-terminus of mouse CLASP1. This sequence is highly conserved in human, rat, chicken, and fish CLASP1, and has low homology to CLASP2. [UniProt# Q80TV8]
• Specificity: The antibody detects a 161 kDa protein corresponding to the molecular mass of CLASP1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects CLASP1 by immunocytochemistry in HeLa cells.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CLASP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500
• Theoretical MW: 161 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for CLASP1 Antibody (KT66) - C-terminus - BSA Free

• CLIP-associating protein 1
• cytoplasmic linker associated protein 1
• Cytoplasmic linker-associated protein 1
• DKFZp686D1968
• DKFZp686H2039
• hOrbit1
• KIAA0622FLJ33821
• MAST1FLJ41222
• MGC131895
• multiple asters 1
• Multiple asters homolog 1
• Protein Orbit homolog 1

Background

CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLASP1