CL-P1/COLEC12 Antibody [Unconjugated]

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CL‑P1/COLEC12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3130, filled histogram) or isotype control antibody (Catalog #AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

• Reactivity: Mu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CL-P1/COLEC12 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CL‑P1/COLEC12
  Ala101-Leu742
  Accession # Q8K4Q8
• Specificity: Detects mouse CL-P1/COLEC12 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Colec12
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CL-P1/COLEC12 Antibody [Unconjugated]

• CL-P1
• CLP1hCL-P1
• COLEC12
• collectin placenta 1
• Collectin placenta protein 1
• collectin sub-family member 12
• collectin-12
• NSR2
• Nurse cell scavenger receptor 2
• SCARA4
• SCARA4NSR2
• Scavenger receptor class A member 4
• scavenger receptor class A, member 4
• SRCL Type I
• SRCLScavenger receptor with C-type lectin

Background

Collectins are a family of Ca ++ -dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1-4). Collectin placenta 1 (CL-P1), also known as collectin sub-family member 12 and scavenger receptor with C-type lectin type I (SRCL), is a 140 kDa member of the collectin family of glycoproteins. With two exceptions, all collectins are secreted. CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Mouse CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that includes an N-terminal 39 aa cytoplasmic domain, an 18 aa transmembrane segment, and a 685 aa C-terminal extracellular domain. The short cytoplasmic domain contains an internalization motif (Y-K-R-F), while the ECD is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure, and a C-type lectin/carbohydrate recognition domain (CRD) (5, 6). Unlike human CL-P1, no splice variants of mouse CL-P1 have been described (5, 7). Trimerization of CL-P1 is mediated by its collagen-like and coiled-coil helical domains (1, 6). Within the ECD, mouse CL-P1 shares 88%, 89%, 92%, and 98% aa sequence identity with bovine, canine, human, and rat CL-P1, respectively. The CRD shares 23-27% aa sequence identity with the CRD of collectins CL-L1, collectin sub-family member 11, MBL, SP-A1, and SP-D. Notably, this CRD recognizes galactose and fucose within the context of asialo-orosomucoids associated with the Lewis x epitope (8, 9). CL-P1 is expressed in vascular endothelial cells and may play a role in bacterial recognition or as a scavenger receptor for desialylated glycoproteins (6, 8).

1. van de Wetering, J.K. et al. (2004) Eur. J. Biochem. 271:1229.
2. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
3. Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
4. Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
5. Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.
6. Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
7. Nakamura, K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
8. Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
9. Yoshida, T. et al. (2003) J. Biochem. 133:271.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CL-P1/COLEC12 Antibody (AF3130)(2)

Publications using AF3130

• JF Bogie, J Mailleux, E Wouters, W Jorissen, E Grajchen, J Vanmol, K Wouters, N Hellings, J Van Horsen, T Vanmierlo, JJ Hendriks Scavenger receptor collectin placenta 1 is a novel receptor involved in the uptake of myelin by phagocytes Sci Rep, 2017-03-20;7(0):44794. 2017-03-20 [PMID: 28317919] (Flow Cytometry, Mouse)
• Tran , Hai B, Ahern , Jessica, Hodge , Greg, Holt , Phillip, Dean , Melinda, Reynolds , Paul N, Hodge , Sandra Oxidative stress decreases functional airway mannose binding lectin in COPD. PLoS ONE, 2014-06-05;9(6):e98571. 2014-06-05 [PMID: 24901869] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Colec12
• Uniprot:
  • Q8K4Q8()