CKII alpha prime polypeptide Antibody - BSA Free

Western Blot: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of Human and Mouse CKII alpha' by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: CKII alpha prime polypeptide Antibody [NB100-379] - Detection of human CKII alpha' by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CKII alpha' antibody NB100-379 used for IP at 6 ug per reaction. CKII alpha' was also immunoprecipitated by rabbit anti-CKII alpha' antibody NB100-378. For blotting immunoprecipitated CKII alpha', NB100-379 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CKII alpha prime polypeptide Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 350 and the C-terminus (residue 350) of human Casein Kinase 2, alpha 2 using the numbering given in entry NP_001887.1 (GeneID 1459).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSNK2A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2- 10 ug/mg lysate
  • Western Blot 1:200- 1:10000
• Application Notes: By Western Blot, this antibody recognizes a band at ~40 kDa, representing CKII alpha'.
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CKII alpha prime polypeptide Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CKII alpha prime polypeptide Antibody - BSA Free

• casein kinase 2, alpha prime polypeptide
• casein kinase II subunit alpha'
• CK II alpha'
• CK2A2
• CKII alpha prime polypeptide
• CSNK2A1
• CSNK2A2
• EC 2.7.11
• EC 2.7.11.1
• FLJ43934

Background

CKII alpha is involved in leukemogensis and tumorigenesis. It is a catalytic, ubiquitous cellular kinase that can use ATP and GTP as phosphorylation donors. CKII alpha also plays a role in many proliferation-related processes in the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CKII alpha prime polypeptide Antibody (NB100-379)(7)

Publications using NB100-379

• Mansky RH, Greguske EA, Yu D et al. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease Cell reports 2023-03-02 [PMID: 36867535] (WB, Mouse)
• Warsinger-Pepe N, Chang C, Desroberts CR et Al. Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible Hsp70Bb promoter in Drosophila melanogaster G3 (Bethesda) 2023-04-13 [PMID: 36705519]
• Yu D, Zarate N, White A et al. CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in huntington's disease Acta neuropathologica communications 2022-06-03 [PMID: 35659303] (WB, Mouse)
• Deshiere A, Duchemin-Pelletier E, Spreux E et al. Unbalanced expression of CK2 kinase subunits is sufficient to drive epithelial-to-mesenchymal transition by Snail1 induction. Oncogene 2012-05-01 [PMID: 22562247]
• Xu J, Reddy BJ, AnP et al. Casein kinase 2 reverses tail-independent inactivation of kinesin-1 Nat Commun 2012-03-27 [PMID: 22453827] (WB, Human)
• Hamdorf M, Berger A, Schule S et al. PKCDelta-induced PU.1 phosphorylation promotes hematopoietic stem cell differentiation to dendritic cells. Stem Cells 2011-02-01 [PMID: 21732487]
• Gao Y, Wang HY. Casein kinase 2 Is activated and essential for Wnt/beta-catenin signaling. J Biol Chem 2006-07-01 [PMID: 16672224]

Bioinformatics

• Gene Symbol: CSNK2A2
• Entrez:
  • 1459(Human)
• Uniprot:
  • P19784(Human)