Casein Kinase 2 beta Antibody (2F12F3) - BSA Free

Western Blot: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis using CSNK2B mouse mAb against Hela (1), Jurkat (2), K562 (3), HepG2 (4), C6 (5), SK-N-SH (6), NTERA-2 (7), MCF-7 (8), NIH/3T3 (9) cell lysate.

Immunocytochemistry/Immunofluorescence: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis of MCF-7 cells using CSNK2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis of paraffin-embedded colon cancer tissues using CSNK2B mouse mAb with DAB staining.

Flow Cytometry: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Flow cytometric analysis of Hela cells using CSNK2B mouse mAb (green) and negative control (red).

Western Blot: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis using CSNK2B mAb against HEK293 (1) and CSNK2B (AA: FULL(1-215))-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis using CSNK2B mAb against human CSNK2B (AA: FULL(1-215)) recombinant protein. (Expected MW is 35 kDa)

Immunohistochemistry: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Analysis of paraffin-embedded cervical cancer tissues using CSNK2B mouse mAb with DAB staining.

ELISA: Casein Kinase 2 beta Antibody (2F12F3) [NBP2-52449] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 2F12F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Casein Kinase 2 beta Antibody (2F12F3) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Casein Kinase 2 beta (AA: FULL(1-215)) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSNK2B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Casein Kinase 2 beta Antibody (2F12F3) - BSA Free

• Casein Kinase 2 beta
• casein kinase 2, beta polypeptide
• Casein kinase II beta subunit
• casein kinase II subunit beta
• CK II beta
• CK2B
• CK2N
• CSK2B
• CSNK2B
• G5A
• MGC138222
• MGC138224
• Phosvitin
• Protein G5a

Background

Casein Kinase 2 beta encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSNK2B