CIN85/SH3KBP1 Antibody (931308) [Unconjugated]

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CIN85/SH3KBP1 Monoclonal Antibody (Catalog # MAB8877) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CIN85/SH3KBP1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 931308
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CIN85/SH3KBP1 Antibody (931308) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CIN85/SH3KBP1
  Pro366-Lys665
  Accession # Q96B97
• Specificity: Detects human CIN85/SH3KBP1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SH3KBP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CIN85/SH3KBP1 Antibody (931308) [Unconjugated]

• C-Cbl-Interacting Protein
• CD2-binding protein 3
• CD2BP3
• CIN85
• CIN85Cbl-interacting protein of 85 kDa
• GIG10
• HSB1
• HSB-1
• Human Src family kinase-binding protein 1
• MIG18
• migration-inducing gene 18
• SH3 domain-containing kinase-binding protein 1
• SH3-domain kinase binding protein 1
• SH3KBP1
• Src family kinase-binding protein 1
• src-related kinase binding protein-1

Background

Independently identified by several groups, SH3-domain kinase-binding protein 1 (SH3KBP1) is also known as CBL-interacting protein of 85 kDa (CIN85), CD2-binding protein 3 (CD2BP3) and human src family kinase-binding protein 1 (HSB1).  SH3KBP1 is a widely expressed adaptor protein involved in regulating diverse signal transduction pathways, including endocytosis, lysosomal degradation, cell adhesion, stress response, cell morphology and cytoskeletal organization.  The protein contains three N-terminal SH3 domains for complex assembly and a C-terminal coiled coil domain for oligomerization, separated by a proline-rich central region.  Smaller known isoforms of SH3KBP1 lack one or more of the SH3 domains.  Over amino acids (aa) 366-665, human SH3KBP1 shares 92% and 90% aa identity with mouse and rat SH3KBP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SH3KBP1
• Uniprot:
  • Q96B97()