CILP-1 Antibody

Immunohistochemistry-Paraffin: CILP-1 Antibody [NBP1-81667] - Staining of human Ovary shows strong positivity in extracellular matrix in follicle cells and stromal cells.

Product Discontinued

CILP-1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: YKHESKLVLRKLQQHQAGEYFCKAQSDAGAVKSKVAQLIVTASDETPCNPVPESYLIRLPHDCFQNATNSFYYDVGRCPVKTCAGQQDNGIRCRDAVQNCCGISKTEEREIQCSGYTLPTK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CILP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35005773). Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CILP-1 Antibody

• cartilage intermediate layer protein 1 C1
• cartilage intermediate layer protein 1 C2
• cartilage intermediate layer protein 1
• cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
• Cartilage intermediate-layer protein
• CILP
• CILP1
• CILP-1
• HsT18872

Background

CILP, Cartilage Intermediate Layer Protein, is specifically expressed in the articular cartilage intermediate layer (it cannot be found in the superficial nor in the deepest layers) and is involved in scaffolding. Overexpression of this protein may lead to impairment of chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Antibodies against CILP are detected in patients with early-stage knee osteoarthritis and defects in this gene may be a cause of susceptibility to lumbar disk disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CILP-1 Antibody (NBP1-81667)(2)

Publications using NBP1-81667

• Lin YL, Yu L, Yan M et al. Induced regeneration of articular cartilage: Identification of a dormant regeneration program for a non-regenerative tissue Development (Cambridge, England) 2023-10-26 [PMID: 37882667] (IHC-P, Mouse)
• Yu L, Lin YL, Yan M et al. Hyaline cartilage differentiation of fibroblasts in regeneration and regenerative medicine Development (Cambridge, England) 2022-01-15 [PMID: 35005773] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: CILP