cIAP-2/HIAP-1 Antibody

Western Blot: cIAP-2/HIAP-1 Antibody - Unpurified [NB100-56131] - cIAP-2/HIAP-1 Antibody [NB100-56131] - Analysis of cIAP-2/HIAP-1 in various cancer cell lines using this antibody at 1:2000.

Immunohistochemistry: cIAP-2/HIAP-1 Antibody - Unpurified [NB100-56131] - cIAP-2/HIAP-1 Antibody [NB100-56131] - Analysis of cIAP-2/HIAP-1 in paraffin-embedded formalin-fixed human mammary gland (female breast) using NB100-56131 at 1:2000. A) normal breast tissue. B) ductal carcinoma in situ (DCIS). C) invasive neoplasia. cIAP2 expressed increased sucessively with tumor progression. Hematoxylin-eosin counterstain.

Product Discontinued

cIAP-2/HIAP-1 Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids 520-570 (KYIPTEDVSDLPVEEQLR) of human cIAP2 was used as immunogen, GenBank no. 2205253B. The sequence used for immunogen is 88% conserved in cow, and 83% conserved in pig, chicken, rat, and mouse.
• Specificity: cIAP2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BIRC3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Control:

  cIAP-2/HIAP-1 Overexpression Lysate

Reactivity Notes

Reacts with Human. 88% sequence identity with bovine and 83% with Chicken, Porcine, Rat and mouse proteins

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for cIAP-2/HIAP-1 Antibody

• AIP1
• API2
• BIRC3 baculoviral IAP repeat containing 3
• BIRC3
• cIAP2
• c-IAP2
• cIAP-2
• HAIP1
• HIAP1
• HIAP-1
• MALT2
• MIHC
• RNF49

Background

cIAP2 (API2, BIRC3, HIAP2, MIHC) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria dependent and independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubiquitination. Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. The IAPs, including cIAP2, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage (see Vischioni et al. 2005 for a comprehensive study). This antibody recognizes cIAP2, human cIAP2 is a 604 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for cIAP-2/HIAP-1 Antibody (NB100-56131)(1)

Publication using NB100-56131

• Schuster C, Malinowsky K, Liebmann S et al. Antibody validation by combining immunohistochemistry and protein extraction from formalin-fixed paraffin-embedded tissues. Histopathology. 2012-05-01 [PMID: 22393911] (IHC-P, WB, Human)
  
  Details:
  cIAP2/BIRC3 (IMG-5718): Formalin-fixed paraffin-embedded human colon carcinoma tissue was used in WB & IHC (paraffin), Tables 1, 2, S1, S2, Fig 3F. cIAP2 was primarily detected at 34 kDa in WB, which is an cIAP2 alternate variant form. Note: The purpose o

Bioinformatics

• Gene Symbol: BIRC3
• Entrez:
  • 330(Human)