Western Blot: cIAP-2/HIAP-1 Antibody [NB100-56131] - Analysis of cIAP-2/HIAP-1 in various cancer cell lines using NB100-56131 at 1:2000.
Immunohistochemistry: cIAP-2/HIAP-1 Antibody [NB100-56131] - Analysis of cIAP-2/HIAP-1 in paraffin-embedded formalin-fixed human mammary gland (female breast) using NB100-56131 at 1:2000. A, normal breast tissue. B, ...read more
WB, IHC, IHC-Fr, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.
A synthetic peptide corresponding to amino acids 520-570 (KYIPTEDVSDLPVEEQLR) of human cIAP2 was used as immunogen, GenBank no. 2205253B. The sequence used for immunogen is 88% conserved in cow, and 83% conserved in pig, chicken, rat, and mouse.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Reacts with Human. 88% sequence identity with bovine and 83% with Chicken, Porcine, Rat and mouse proteins
Alternate Names for cIAP-2/HIAP-1 Antibody
BIRC3 baculoviral IAP repeat containing 3
cIAP2 (API2, BIRC3, HIAP2, MIHC) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination. Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. The IAPs, including cIAP2, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage (see Vischioni et al. 2005 for a comprehensive study). This antibody recognizes cIAP2, human cIAP2 is a 604 amino acid protein.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.