Chordin-like 2/CHRDL2 Antibody

Chordin-like 2/CHRDL2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human Chordin-like 2/CHRDL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2448) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Chordin-like 2/CHRDL2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Chordin-like 2/CHRDL2
  Arg28-Thr451
  Accession # NP_056239
• Specificity: Detects human Chordin-like 2/CHRDL2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHRDL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chordin-like 2/CHRDL2 Antibody

• BNF-1
• Chordin like 2
• Chordin-like 2
• chordin-like protein 2
• CHRDL2

Background

CHRDL2, also known as breast tumor novel factor 1 (BNF-1), is a secreted BMP antagonist implicated in chondrocyte development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHRDL2
• Uniprot:
  • NP_056239()