Choline Acetyltransferase/ChAT Antibody

Product Discontinued

Choline Acetyltransferase/ChAT Antibody Summary

• Immunogen: Native choline acetyltransferase purifed from human placenta
• Marker: Cholinergic Neuronal Marker
• Specificity: Specific for the ~ 68/70 k choline acetyltransferase protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHAT
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
• Application Notes: Useful in Immunofluorescence (PMID: 21468686).
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for Choline Acetyltransferase/ChAT Antibody

• acetyl CoA:choline O-acetyltransferase
• CHAT
• CHOACTase
• Choline acetylase
• Choline Acetyltransferase
• choline O-acetyltransferase
• CMS1A
• CMS1A2
• EC 2.3.1
• EC 2.3.1.6

Background

Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Choline Acetyltransferase/ChAT Antibody (NB110-93361)(2)

Publications using NB110-93361

• Burugula B, Ganesh B, Chintala S et al. Curcumin attenuates staurosporine-mediated death of retinal ganglion cells Invest Ophthalmol Vis Sci 2011-06-16 [PMID: 21498608] (IF/IHC, ICC/IF, Mouse)
• Raats DA, de Bruijn MT, Steller EJ et al. Synergistic killing of colorectal cancer cells by oxaliplatin and ABT-737. Cell Oncol (Dordr). 2011-08-01 [PMID: 21468686]

Bioinformatics

• Gene Symbol: CHAT
• Entrez:
  • 1103(Human)
  • 12647(Mouse)
  • 290567(Rat)
• Uniprot:
  • P28329.(Human)