Choline Acetyltransferase/ChAT Antibody

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Western Blot: Choline Acetyltransferase/ChAT Antibody [NB100-40794] - Analysis of Choline Acetyltransferase (mouse) using this antibody at 0.2 ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).
Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody [NB100-40794] - Staining of PFA-perfused cryosection of Mouse Basal Forebrain. HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained ...read more
Western Blot: Choline Acetyltransferase/ChAT Antibody [NB100-40794] - Choline Acetyltransferase Antibody [NB100-40794] - (0.2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, IHC, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Choline Acetyltransferase/ChAT Antibody Summary

Immunogen
Peptide with sequence C-KERARGPTKPSNLD corresponding to C-Terminus according to Uniprot Mouse NP_034021.1.
Marker
Cholinergic Neuronal Marker
Specificity
This antibody is NOT expected to cross-react to Human CHAT.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 0.5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 75-80 kDa band observed in mouse and rat brain lysates (calculated MW of 72.3 kDa band according to NP_034021.1).
Publications
Read Publication using NB100-40794.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Choline Acetyltransferase/ChAT Antibody

  • acetyl CoA:choline O-acetyltransferase
  • CHAT
  • CHOACTase
  • Choline acetylase
  • Choline Acetyltransferase
  • choline O-acetyltransferase
  • CMS1A
  • CMS1A2
  • EC 2.3.1
  • EC 2.3.1.6

Background

Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. Dysfunction of cholinergic neurons underlies aspects of clinical symptoms found in neurological and psychiatric disorders such as Alzheimer's disease, Down and Rett syndromes. Choline acetyltransferase recruits choline for the synthesis of acetylcholine at preganglionic parasympathetic and sympathetic fibres. It is also present at parasympathetic postganglionic fibres.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Choline Acetyltransferase/ChAT Antibody (NB100-40794)(1)

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Blogs on Choline Acetyltransferase/ChAT.

Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons
Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group...  Read full blog post.

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Bioinformatics

Gene Symbol CHAT
Entrez
Uniprot