Choline Acetyltransferase/ChAT Antibody

Western Blot: Choline Acetyltransferase/ChAT Antibody [NB100-40794] - Staining of mouse (A) and rat (B) brain lysate. Antibody at 1 ug/mL, 35 ug protein in RIPA buffer. Detected by chemiluminescence.

Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody [NB100-40794] - Staining of PFA-perfused cryosection of Mouse Basal Forebrain. HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

Product Discontinued

Choline Acetyltransferase/ChAT Antibody Summary

• Immunogen: Peptide with sequence C-KERARGPTKPSNLD corresponding to C-Terminus according to Uniprot Mouse NP_034021.1.
• Marker: Cholinergic Neuronal Marker
• Specificity: This antibody is NOT expected to cross-react to Human CHAT.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHAT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.1 - 0.3ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.2 - 0.5 ug/mL
• Application Notes: WB: Approx. 75-80 kDa band observed in mouse and rat brain lysates (calculated MW of 72.3 kDa band according to NP_034021.1).

Reactivity Notes

Expected from sequence similarity: Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Choline Acetyltransferase/ChAT Antibody

• acetyl CoA:choline O-acetyltransferase
• CHAT
• CHOACTase
• Choline acetylase
• Choline Acetyltransferase
• choline O-acetyltransferase
• CMS1A
• CMS1A2
• EC 2.3.1
• EC 2.3.1.6

Background

Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. Dysfunction of cholinergic neurons underlies aspects of clinical symptoms found in neurological and psychiatric disorders such as Alzheimer's disease, Down and Rett syndromes. Choline acetyltransferase recruits choline for the synthesis of acetylcholine at preganglionic parasympathetic and sympathetic fibres. It is also present at parasympathetic postganglionic fibres.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Choline Acetyltransferase/ChAT Antibody (NB100-40794)(1)

Publication using NB100-40794

• Contestabile A, Fila T, Bartesaghi R et al. Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases. J Neurochem 2006-04-01 [PMID: 16539660]

Bioinformatics

• Gene Symbol: CHAT
• Entrez:
  • 12647(Mouse)
  • 432359(Rat)
• Uniprot:
  • NP_034021.1(Mouse)