CHMP5 Antibody

Images

 
Western Blot: CHMP5 Antibody [NBP1-32597] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 2% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CHMP5 Antibody [NBP1-32597] - HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: CHMP5 Antibody [NBP1-32597] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.
Western Blot: CHMP5 Antibody [NBP1-32597] - Sample (50 ug of whole cell lysate) A: mouse stomach 12% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.7 mg/ml

Order Details

CHMP5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CHMP5. The exact sequence is proprietary.
Localization
Cytoplasm, cytosol, Endosome membrane, Peripheral membrane protein, Cytoplasm, cytosol, Endosome membrane
Predicted Species
Rat (98%), Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHMP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.7 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CHMP5 Antibody

  • apoptosis-related protein PNAS-2
  • C9orf83
  • charged multivesicular body protein 5
  • chromatin modifying protein 5
  • Chromatin-modifying protein 5
  • chromosome 9 open reading frame 83
  • HSPC177
  • hVps60
  • PNAS-2
  • SNF7 domain containing 2
  • SNF7 domain-containing protein 2
  • SNF7DC2
  • Vacuolar protein sorting-associated protein 60
  • Vps60CGI-34

Background

CHMP5 is a component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargoes of viral proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHMP5