Chk2 Antibody (1238H) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 1238H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

Chk2 Antibody (1238H) [Alexa Fluor® 532] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E.coli-derived recombinant human Phospho-Chk2 (T68)
  Accession # O96017
• Specificity: Detects human Chk2 when phosphorylated at T68 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chk2 Antibody (1238H) [Alexa Fluor® 532]

• CDS1
• CHEK2
• CHK2 checkpoint homolog (S. pombe)
• Chk2
• EC 2.7.11
• EC 2.7.11.1
• HuCds1
• LFS2
• PP1425
• Rad53
• S.pombe) homolog

Background

Serine/threonine-protein kinase Chk2 (CHEK2) is a 65 kDa member of Serine/threonine-protein kinase family of proteins. Chk2 regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Chk2 is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks.Existence of multiple splice variants of Chk2 is predicted. Human Chk2 shares 86% aa sequence identity with mouse Chk2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.