Chitotriosidase/CHIT1 Antibody

Western Blot: CHIT1 Antibody [NBP1-97774] - (1ug/ml) Analysis of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Chitotriosidase/CHIT1 Antibody Summary

• Immunogen: Peptide with sequence EHGPSPGQDTFCQGK corresponding to internal region (near C-Terminus) according to NP_003456.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHIT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in Daudi lysates (calculated MW of 51.7 kDa band according to NP_003456.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Chitotriosidase/CHIT1 Antibody

• CHI3
• CHIT1
• CHITCHI3
• chitinase 1 (chitotriosidase)
• chitinase-1
• Chitotriosidase
• chitotriosidase-1
• EC 3.2.1.14
• FLJ00314
• MGC125322
• plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

Background

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chitotriosidase/CHIT1 Antibody (NBP1-97774)(1)

Publication using NBP1-97774

• Chien YH, Lee NC, Tsai FJ et al. Reduction in imiglucerase dosage causes immediate rise of chitotriosidase activity in patients with Gaucher disease. Mol Genet Metab 2010-09-01 [PMID: 20580583]

Bioinformatics

• Gene Symbol: CHIT1
• Entrez:
  • 1118(Human)
• Uniprot:
  • NP_003456.1(Human)