CHFR Antibody

Immunohistochemistry-Paraffin: CHFR Antibody [NB100-1166] - IHC analysis of CHFR in paraffin-embedded Human Brain (Cerebellum) using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CHFR Antibody Summary

• Description: Novus Biologicals Goat CHFR Antibody (NB100-1166) is a polyclonal antibody validated for use in IHC and ELISA. Anti-CHFR Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence KFNHICEQTRFKN corresponding to C-Terminus according to NP_001154817.1, NP_001154818.1, NP_060693.2, NP_001154819.1.
• Specificity: This antibody is expected to recognise all reported isoforms.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHFR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave an approx. 80 kDa band in human brain lysates at 0.5 ug/ml, this band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in scientific literature for the band we observe given the predicted MW of approx. 72 kDa according to NP_060693. IHC-P: Human brain (cerebellum) shows stained Purkinje cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CHFR Antibody

• Checkpoint with forkhead and RING finger domains protein
• checkpoint with forkhead and ring finger domains
• E3 ubiquitin-protein ligase CHFR
• EC 6.3.2.-
• FLJ10796
• FLJ33629
• RING finger protein 196
• RNF196RNF116

Background

A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45% of cancer cell lines, 40% of primary colorectal cancers, 53% of colorectal adenomas, and 30% of primary head and neck cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHFR Antibody (NB100-1166)(1)

Publication using NB100-1166

• Scolnick DM, Halazonetis TD. Chfr defines a mitotic stress checkpoint that delays entry into metaphase. Nature 2000-07-27 [PMID: 10935642]

Bioinformatics

• Gene Symbol: CHFR
• Entrez:
  • 55743(Human)
  • 231600(Mouse)
  • 288734(Rat)
• Uniprot:
  • NP_060693.(Human)