Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse liver using ChemR23/CMKLR1 antibody (clone BZ194) at 1:200 ...read more
This antibody was tested in Flow on resident mouse peritoneal macrophages and found that it can be used at less than or equal to 0.5 ug per test (cell number should be determined empirically but can range from 10^5 to 10^8cells/test). In IHC-P it was tested on mouse liver using routine protocol with heat induced antigen retrieval employing 10mM sodium citrate buffer pH 6.
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Alternate Names for ChemR23/CMKLR1 Antibody (BZ194) - BSA Free
chemokine receptor-like 1
chemokine-like receptor 1
G-protein coupled receptor ChemR23
G-protein coupled receptor DEZ
orphan G-protein coupled receptor, Dez
The 194.7.4 (BZ194)monoclonal antibody reacts to mouse Chemoattractant-like receptor 1 (CMKLR1), also known as ChemR23 and DEZ. CMKLR1 is the receptor for chemerin, a potent chemoattractant. The BZ194 antibody was generated against the amino terminal residues of mouse CMKLR1. In the human CMKLR is found on pDC while in the mouse expression is confined to resident peritoneal macrophages with highest expression on Cd11blo macrophages. Expression is absent on in vitro cultured bone marrow DCs (except on early DCs as defined by CD11c+MHCIIint), and on freshly isolated CD11c+ cells from lymph node, spleen and bone marrow. Mouse CMKLR is thought to play a role in macrophage function as a result of injury.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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