ChemR23/CMKLR1 Antibody (BZ194)

Images

 
Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse liver using ChemR23/CMKLR1 antibody (clone BZ194) at 1:200 ...read more
Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - Staining of resident mouse peritoneal macrophages.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-P
Clone
BZ194
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

ChemR23/CMKLR1 Antibody (BZ194) Summary

Immunogen
The immunogen for this antibody was CMKLR1.
Localization
Multi-pass membrane protein with cytoplasmic and extra-cellular domains
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CMKLR1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
Application Notes
For FLOW validation, we tested this antibody on resident mouse peritoneal macrophages and found that it can be used at less than or equal to 0.5 ug per test (cell number should be determined empirically but can range from 10^5 to 10^8cells/test). In IHC-P, we tested it on mouse liver using routine protocol with heat induced antigen retrieval employing 10mM sodium citrate buffer pH 6
Publications
Read Publication using
NBP1-43233 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for ChemR23/CMKLR1 Antibody (BZ194)

  • CHEMERINR
  • chemokine receptor-like 1
  • chemokine-like receptor 1
  • ChemR23
  • cmklr1
  • Dez
  • Gpcr27
  • G-protein coupled receptor ChemR23
  • G-protein coupled receptor DEZ
  • MGC126105
  • MGC126106
  • orphan G-protein coupled receptor, Dez

Background

The 194.7.4 (BZ194)monoclonal antibody reacts to mouse Chemoattractant-like receptor 1 (CMKLR1), also known as ChemR23 and DEZ. CMKLR1 is the receptor for chemerin, a potent chemoattractant. The BZ194 antibody was generated against the amino terminal residues of mouse CMKLR1. In the human CMKLR is found on pDC while in the mouse expression is confined to resident peritoneal macrophages with highest expression on Cd11blo macrophages. Expression is absent on in vitro cultured bone marrow DCs (except on early DCs as defined by CD11c+MHCIIint), and on freshly isolated CD11c+ cells from lymph node, spleen and bone marrow. Mouse CMKLR is thought to play a role in macrophage function as a result of injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ChemR23/CMKLR1 Antibody (NBP1-43233)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

7C Labeled NBP1-43233V2
Alexa Fluor 405 Labeled NBP1-43233AF405
Alexa Fluor 488 Labeled NBP1-43233AF488
Alexa Fluor 647 Labeled NBP1-43233AF647
Alexa Fluor 700 Labeled NBP1-43233AF700
Allophycocyanin Labeled NBP1-43233APC
DyLight 350 Labeled NBP1-43233UV
DyLight 405 Labeled NBP1-43233V
DyLight 405LS Labeled NBP1-43233V3
DyLight 488 Labeled NBP1-43233G
DyLight 680 Labeled NBP1-43233FR
DyLight 755 Labeled NBP1-43233IR
PE Labeled NBP1-43233PE
PerCP Labeled NBP1-43233PCP

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Bioinformatics

Gene Symbol CMKLR1
Entrez
Uniprot