ChemR23/CMKLR1 Antibody (BZ194) - BSA Free

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Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse liver using ChemR23/CMKLR1 antibody (clone BZ194) at 1:200 ...read more
Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) [NBP1-43233] - Staining of resident mouse peritoneal macrophages.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-P
Clone
BZ194
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

ChemR23/CMKLR1 Antibody (BZ194) - BSA Free Summary

Immunogen
Partial synthetic peptide made to an N-terminal portion of the mouse ChemR23/CMKLR1 protein (amino acids 8-32) [UniProt P97468]
Localization
Multi-pass membrane protein with cytoplasmic and extra-cellular domains
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CMKLR1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
Application Notes
This antibody was tested in Flow on resident mouse peritoneal macrophages and found that it can be used at less than or equal to 0.5 ug per test (cell number should be determined empirically but can range from 10^5 to 10^8cells/test). In IHC-P it was tested on mouse liver using routine protocol with heat induced antigen retrieval employing 10mM sodium citrate buffer pH 6.
Publications
Read Publications using
NBP1-43233 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for ChemR23/CMKLR1 Antibody (BZ194) - BSA Free

  • CHEMERINR
  • chemokine receptor-like 1
  • chemokine-like receptor 1
  • ChemR23
  • cmklr1
  • Dez
  • Gpcr27
  • G-protein coupled receptor ChemR23
  • G-protein coupled receptor DEZ
  • MGC126105
  • MGC126106
  • orphan G-protein coupled receptor, Dez

Background

The 194.7.4 (BZ194)monoclonal antibody reacts to mouse Chemoattractant-like receptor 1 (CMKLR1), also known as ChemR23 and DEZ. CMKLR1 is the receptor for chemerin, a potent chemoattractant. The BZ194 antibody was generated against the amino terminal residues of mouse CMKLR1. In the human CMKLR is found on pDC while in the mouse expression is confined to resident peritoneal macrophages with highest expression on Cd11blo macrophages. Expression is absent on in vitro cultured bone marrow DCs (except on early DCs as defined by CD11c+MHCIIint), and on freshly isolated CD11c+ cells from lymph node, spleen and bone marrow. Mouse CMKLR is thought to play a role in macrophage function as a result of injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ChemR23/CMKLR1 Antibody (NBP1-43233)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, IHC.


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ICC/IF
(1)
IHC
(1)
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(2)
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Bioinformatics

Gene Symbol CMKLR1
Entrez
Uniprot