CHD2 Antibody

Western Blot: CHD2 Antibody [NBP1-33428] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CHD2 antibody [N1N2], N-term diluted at 1:1000.

Immunohistochemistry-Paraffin: CHD2 Antibody [NBP1-33428] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.

Product Discontinued

CHD2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CHD2. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (97%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 211 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for CHD2 Antibody

• ATP-dependent helicase CHD2
• CHD-2
• chromodomain helicase DNA binding protein 2
• chromodomain-helicase-DNA-binding protein 2
• DKFZp547I1315
• DKFZp686E01200
• DKFZp781D1727
• EC 3.6.1
• EC 3.6.4.12
• FLJ38614

Background

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CHD2