CHCHD4 Antibody

Western Blot: CHCHD4 Antibody [NBP2-76390] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHCHD4 antibody NBP2-76390 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: CHCHD4 Antibody [NBP2-76390] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHCHD4 antibody NBP2-76390 used for IP at 100 ul per reaction. CHCHD4 was also immunoprecipitated by rabbit anti-CHCHD4 antibody NBP2-76389. For blotting immunoprecipitated CHCHD4, NBP2-76390 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.

Product Discontinued

CHCHD4 Antibody Summary

• Immunogen: Immunogen sequence between amino acids 92 and 142
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHCHD4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 50-100 ul/mg lysate
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Tris-buffered Saline containing 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for CHCHD4 Antibody

• CHDH4
• coiled-coil-helix-coiled-coil-helix domain containing 4
• Coiled-coil-helix-coiled-coil-helix domain-containing protein 4
• FLJ31709
• MIA40mitochondrial intermembrane space import and assembly protein 40
• mitochondrial intermembrane space import and assembly 40 homolog
• TIMM40
• translocase of inner mitochondrial membrane 40 homolog

Background

CHCHD4 functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19 and MICU1. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system. Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHCHD4 Antibody (NBP2-76390)(1)

Publication using NBP2-76390

• Bruhn C, Bastianello G, Foiani M Cancer cell histone density links global histone acetylation, mitochondrial proteome and histone acetylase inhibitor sensitivity Communications biology 2022-08-27 [PMID: 36030322] (WB, Human)
  
  Details:
  Dilution used 1:1000

Bioinformatics

• Gene Symbol: CHCHD4