ch-TOG Antibody

Western Blot: ch-TOG Antibody [NBP1-06513] - Western Blot and Immunoprecipitation: ch-TOG antibody [NBP1-06513]- Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ch-TOG antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ch-TOG was also immunoprecipitated by rabbit anti-ch-TOG antibodies NBP1-06511and NBP1-06512, which recognize upstream epitopes.

• Reactivity: Hu, Mu, Am, Xp
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ch-TOG Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1982 and 2032 of human colonic and hepatic tumor over-expressed protein using the numbering given in entry NP_001008938.1 (GeneID 9793).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CKAP5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 29851557). Amphibian reactivity reported in scientific literature (PMID:29851557).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ch-TOG Antibody

• CHTOG
• Ch-TOG
• Colonic and hepatic tumor over-expressed gene protein
• cytoskeleton associated protein 5
• cytoskeleton-associated protein 5
• EC 2.7.7.6
• EC 3.1.1.29
• KIAA0097FLJ35359
• MSPS
• TOG
• TOGp

Background

Colonic and hepatic tumor over-expressed protein (ch-TOG) is a protein originally described as a gene over-expressed in human hepatomas and colonic tumors. It was found to be a homolog of the xenopus egg protein, XMAP214, a microtubule associated protein (MAP). The human ch-TOG protein has been shown to promote microtubule assembly and is a major component of microtubule asters. It is required for centrosome integrity, spindle pole organization and bipolar spindle assembly. Alternative names for ch-TOG include cytoskeleton-associated protein 5, CKAP5, TOG, MSPS, TOGp, and KIAA0097.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ch-TOG Antibody (NBP1-06513)(1)

Publication using NBP1-06513

• Milunovic-Jevtic A, Jevtic P, Levy DL et al. In vivo mitotic spindle scaling can be modulated by changing the levels of a single protein: the microtubule polymerase XMAP215 Mol Biol Cell. [PMID: 29851557] (WB, Amphibian)

Bioinformatics

• Gene Symbol: CKAP5
• Entrez:
  • 9793(Human)
  • 75786(Mouse)
• Uniprot:
  • Q14008(Human)