CETP Antibody (995234) [Unconjugated]

CETP was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CETP Monoclonal Antibody (Catalog # MAB10042) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 995234
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CETP Antibody (995234) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CETP
  Cys18-Ser493
  Accession # P11597
• Specificity: Detects human CETP in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CETP Antibody (995234) [Unconjugated]

• BPIFF
• CETP
• cholesteryl ester transfer protein
• cholesteryl ester transfer protein, plasma
• HDLCQ10
• Lipid Transfer Protein I

Background

Cholesteryl Ester Transfer Protein (CETP), also known as plasma lipid transfer protein, is 493 amino acids (aa) secreted plasma protein involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles.  CETP allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P11597()