Human Ceruloplasmin ELISA Kit (Colorimetric)

ELISA: Human Ceruloplasmin ELISA Kit (Colorimetric) [NBP2-60616] - Representative standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture, Urine, Saliva, Milk, CSF
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.12 ng/mL (example only; lot dependent)

Human Ceruloplasmin ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.12 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10.10 (example only; lot dependent)
• Intra-Assay: %CV < 4.40 (example only; lot dependent)
• Spike Recovery: 97% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CP

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Ceruloplasmin ELISA Kit (Colorimetric)

• ceruloplasmin (ferroxidase)
• ceruloplasmin
• CP-2
• EC 1.16.3.1
• Ferroxidase

Background

The protein encoded by the Ceruloplasmin gene is a metalloprotein that binds most of the copper in plasma and is involved in theperoxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, whichresults in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Ceruloplasmin ELISA Kit (NBP2-60616)(4)

Publications using NBP2-60616

• Alan Zanardi, Ilaria Nardini, Sara Raia, Antonio Conti, Barbara Ferrini, Patrizia D’Adamo, Enrica Gilberti, Giuseppe DePalma, Sara Belloli, Cristina Monterisi, Angela Coliva, Paolo Rainone, Rosa Maria Moresco, Filippo Mori, Giada Zurlo, Carla Scali, Letizia Natali, Annalisa Pancanti, Pierangelo Giovacchini, Giulio Magherini, Greta Tovani, Laura Salvini, Vittoria Cicaloni, Cristina Tinti, Laura Tinti, Daniele Lana, Giada Magni, Maria Grazia Giovannini, Alessandro Gringeri, Andrea Caricasole, Massimo Alessio New orphan disease therapies from the proteome of industrial plasma processing waste- a treatment for aceruloplasminemia Communications Biology 2024-01-30 [PMID: 38291108]
• Xie Y, Zhou W, Tao X et al. Early Gestational Blood Markers to Predict Preeclampsia Complicating Gestational Diabetes Mellitus Diabetes, metabolic syndrome and obesity : targets and therapy 2023-05-22 [PMID: 37252009] (ELISA)
• Kim H, Im I, Jeon JS et al. Development of human pluripotent stem cell-derived hepatic organoids as an alternative model for drug safety assessment Biomaterials 2022-05-16 [PMID: 35598335] (ELISA, Human)
• Bailey Dk, Clark W, Kosman Dj The iron chelator, PBT434, modulates transcellular iron trafficking in brain microvascular endothelial cells PloS one 2021-07-26 [PMID: 34310628] (ELISA)

FAQs for Ceruloplasmin ELISA Kit (NBP2-60616). (Showing 1 - 2 of 2 FAQs).

1. We want to set up a Ceruloplasmin enzyme immunoassay. Could you suggest a suitable/optimal antibody pair for a sandwich format?
   • Currently we do not carry any validated antibody pairs for sandwich ELISA to detect ceruloplasmin, however we do have ELISA kits that have been optimized and validated for human and rat.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CP