Cerebellin-2 Antibody (704330)

Western blot shows lysates of human brain (cerebellum) tissue, human brain (cortex) tissue, human brain (hippocampus) tissue, human brain (hypothalamus) tissue, and mouse brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cerebellin-2 Monoclonal Antibody (Catalog # MAB7044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cerebellin-2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 704330
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cerebellin-2 Antibody (704330) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Cerebellin-2
  Arg50-Leu224
  Accession # Q8IUK8
• Specificity: Detects human Cerebellin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cerebellin-1, -3, or -4 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CBLN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cerebellin-2 Antibody (704330)

• CBLN2
• cerebellin 2 precursor
• Cerebellin2
• Cerebellin-2

Background

Cerebellin-2 (CBLN2) is an approximately 25 kDa glycoprotein in the Cerebellin family of C1q- and TNF-related proteins. It contains a potential membrane-associating segment but may also be secreted. It contains one C1q-like domain in its extracellular region. Cerebellin-2 expression is developmentally regulated in the cerebellum, neocortex, sensory neurons of the dorsal root ganglion and spinal cord, and adrenal cortex. It associates with Cerebellin-1 which is involved in the formation and maintenance of synapses in the cerebellum. Within the ectodomain (aa 50-224), human Cerebellin-2 shares 99% aa sequence identity with mouse and rat Cerebellin-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBLN2
• Uniprot:
  • Q8IUK8()