CELA1 Antibody

Images

 
Western Blot: CELA1 Antibody [NBP1-78085] - Lane 1: Elastase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Elastase Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at ...read more
Immunocytochemistry/ Immunofluorescence: CELA1 Antibody [NBP1-78085] - Double immunofluorescence microscopy after staining of mouse embryonic gut tissue with anti-Elastase antibody and anti-Insulin antibody. Tissue ...read more

Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CELA1 Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Elastase [Porcine Pancreas]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CELA1
Purity
Ion exchange chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:3000
  • ELISA 1:5000 - 1:25000
  • Immunocytochemistry/Immunofluorescence
Application Notes
This product has been assayed against 1.0 ug of Elastase [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:1,000 - 1:5,400 is suggested for this product. An investigator has reported that this antibody works very well on sections of mouse embryonic pancreas tissue, specifically staining developing exocrine cells. The investigator stained frozen sections cut from fixed tissue. The detection of antigen in paraffin-embedded samples remains undetermined.

Reactivity Notes

Reactive in swine

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for CELA1 Antibody

  • chymotrypsin-like elastase family member 1
  • chymotrypsin-like elastase family, member 1
  • EC 3.4.21
  • EC 3.4.21.36
  • ELA1elastase-1
  • elastase 1, pancreatic
  • Elastase-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CELA1