CELA1 Antibody - BSA Free

Western Blot: CELA1 Antibody [NBP1-78085] - Lane 1: Elastase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Elastase Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 minutes at RT. Block: incubated with blocking buffer for 30 minutes at RT. Predicted/Observed size: 29 kDa, 29 kDa for Elastase.

Immunocytochemistry/Immunofluorescence: CELA1 Antibody [NBP1-78085] - Double immunofluorescence microscopy after staining of mouse embryonic gut tissue with anti-Elastase antibody and anti-Insulin antibody. Tissue sections were prepared using frozen sections after fixation for 1 h in 4% PFA. The image shows elastase staining in green and insulin staining in red.

• Reactivity: Mu, Po
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

CELA1 Antibody - BSA Free Summary

• Description: This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: CELA1 [Porcine Pancreas] (Uniprot: P00772)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CELA1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:500-1:3000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-1:3000
• Application Notes: This product has been tested by ELISA, western blot, and immunofluorescence. This product is assayed against 1.0 ug of Elastase [Porcine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,400 is suggested for This product An investigator has reported that this antibody works very well on sections of mouse embryonic pancreas tissue, specifically staining developing exocrine cells.

Reactivity Notes

Cross reactivity was reported for mouse CELA1 (mouse embryonic pancreas). Cross reactivity against CELA1 from other tissues and species has not been determined

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for CELA1 Antibody - BSA Free

• chymotrypsin-like elastase family member 1
• chymotrypsin-like elastase family, member 1
• EC 3.4.21
• EC 3.4.21.36
• ELA1elastase-1
• elastase 1, pancreatic
• Elastase-1

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CELA1
• Uniprot:
  • P00772(Porcine)