Recombinant Human CEBP alpha Protein

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SDS-Page: CEBP Alpha Protein [NBC1-18419] - C/EBP alpha, 14.5kDa (126aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human CEBP alpha Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 270-358 of Human CEBP alpha

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGAG KAKKSVDKNS NEYRVRRERN NIAVRKSRDK AKQRNVETQQ KVLELTSDND RLRKRVEQLS RELDTLRGIF RQLPESSLVK AMGNCA

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CEBPA
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl pH7.5, 0.1M NaCl, 5mM beta-Mercaptoethanol.
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human CEBP alpha Protein

  • C/EBP alpha
  • C/EBP-alpha
  • CCAAT/enhancer binding protein (C/EBP), alpha
  • CCAAT/enhancer-binding protein alpha
  • CEBP alpha
  • CEBP
  • CEBPA

Background

CCAAT/enhancer binding protein(C/EBP) alpha is a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP family consist of several related proteins, C/EBP alpha, beta, gamma,delta, that form homodimers and that form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein; a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. C/EBPs differ significantly in their physiological functions and in their downstream target genes. For example, mice lacking C/EBP alpha die shortly after birth due to severe hypoglycemia and the absence of glycogen storage in liver, whereas knockout of C/EBPbeta causes defects in female reproduction The bZip region of CEBP-alpha (residues 270-358)was produced in E.coli and purified by ion-exchange chromatography and FPLC gel-filtration chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for CEBP alpha Protein (NBC1-18419). (Showing 1 - 1 of 1 FAQ).

  1. Do any of your recombinant proteins have DNA-binding activity? I need this as a control for some EMSAs I am doing to confirm my results obtained using nuclear extract.
    • NBC1-18419 is not expected to have DNA binding activity. The buffer is 20mM Tris-HCl pH7.5, 0.1M NaCl, 5mM beta-Mercaptoethanol and this will almost certainly denature the protein. It is intended to be used as a positive control. Unfortunately, we do not carry an active CEBP alpha protein at this time.

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Transcription Factor Antibodies Used In Landmark Evolutionary Study
We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising...  Read full blog post.

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Bioinformatics

Gene Symbol CEBPA
Entrez