CEACAM8/CD66b Antibody (80H3)

Flow Cytometry: CEACAM8/CD66b Antibody (80H3) [NB100-64916] - Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD16) and purified Mouse IgG1 isotype control MCA928) detected with Goat anti Mouse IgG1 FITC ). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD16 ) and purified Mouse anti Human CD66b ) detected with Goat anti Mouse IgG1 FITC /a>). All experiments performed on human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer

Product Discontinued

CEACAM8/CD66b Antibody (80H3) Summary

• Immunogen: Human leucocytes from individuals with CML
• Specificity: NB100-64916 reacts with the CGM6 gene product of the carcinoembryonic gene family. This is a 95-100kD GPI anchored molecule. CEACAM8 is expressed strongly by mature granulocytes and metamyelocytes but weakly by bone marrow myelocytes. CEACAM8 is not expressed by peripheral blood monocytes or lymphocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:25-1:100
  • Western Blot 1:100-1:2000
• Application Notes: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: <0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for CEACAM8/CD66b Antibody (80H3)

• Carcinoembryonic antigen CGM6
• carcinoembryonic antigen gene family member 6
• carcinoembryonic antigen-related cell adhesion molecule 8
• CD66b antigen
• CD66b
• CD67 antigen
• CD67
• CEACAM8
• CEACAM-8
• CGM6
• NCA-95
• Non-specific cross-reacting antigen NCA-95

Background

CEACAM8 is expressed in leukocytes of Chronic Myeloid Leukemia patients and bone marrow. It belongs to the immunoglobulin superfamily, CEA family. The antigen is expressed strongly by mature granulocytes and meta myelocytes, but only weakly by bone marrow myelocytes. Peripheral blood monocytes and lymphocytes do not express significant amounts of the antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEACAM8/CD66b Antibody (NB100-64916)(1)

Publication using NB100-64916

• Wong KHK, Tessier SN, Miyamoto DT et al. Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells Nat Commun. 2017-11-23 [PMID: 29170510] (Human)

FAQs for CEACAM8/CD66b Antibody (NB100-64916). (Showing 1 - 1 of 1 FAQ).

1. What is the average recovery rate for labeling biotin molecules to a purified antibody?
   • The average recovery rate for labeling biotin molecules to any purified antibody is above 75%

Bioinformatics

• Gene Symbol: CEACAM8
• Entrez:
  • 1088(Human)
• Uniprot:
  • P31997()
  • P31997()