CEACAM1/CD66a Antibody (BLR032F)

Western Blot: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 by WB of UACC-257, SK-MEL-28, Hep-G2, SUP-M2, HeLa, HEK293T, Jurkat, RAW 264.7, and TCMK-1 lysate. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (NBP2-76405). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-Actin.

Immunocytochemistry: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 in FFPE HT-29 cells by ICC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (NBP2-76405). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 in FFPE colon carcinoma by ICC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] (NBP2-76405). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

CEACAM1/CD66a Antibody (BLR032F) [NBP2-76405] - Detection of human CEACAM1 by WB of immunoprecipitates from UACC-257 lysate. Antibodies: Rabbit anti-CEACAM1 recombinant monoclonal [BLR032F] NBP2-76405 and rabbit anti-CEACAM1 recombinant monoclonal.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: BLR032F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CEACAM1/CD66a Antibody (BLR032F) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein representing the extracellular domain of human CEACAM1.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CEACAM1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 to 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 20 ul/1 mg lysate
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for CEACAM1/CD66a Antibody (BLR032F)

• antigen CD66
• BGP1
• BGP-1
• BGP1BGPBiliary glycoprotein 1
• BGPI
• Biliary Glycoprotein 1
• biliary glycoprotein adhesion molecule
• carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein)
• carcinoembryonic antigen-related cell adhesion molecule 1
• CD66a antigen
• CD66a
• Cea-1
• CEACAM1
• CEACAM-1
• Hv-1
• Hv-2
• MHVR

Background

Carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1) is a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. CEACAM1 mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to CEACAM1 including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEACAM1