CEACAM1/CD66a Antibody (103) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CEACAM1/CD66a Antibody (103) [NBP2-89666] - Staining of CEACAM1 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CEACAM1 monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).

Flow Cytometry: CEACAM1/CD66a Antibody (103) [NBP2-89666] - Analysis of Human CEACAM1(CD66a) expression in HT29 cells. The cells were harvested and stained with purified Rabbit anti-human CEACAM1, then a FITC-conjugated second step antibody. Flow cytometry was performed on a BD (TM) FACSCalibur flow cytometry system.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 103
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CEACAM1/CD66a Antibody (103) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human CEACAM1/CD66a (Accession#: NP_001020083.1; Met1-Gly428).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CEACAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CEACAM1/CD66a Antibody (103) - Azide and BSA Free

• antigen CD66
• BGP1
• BGP-1
• BGP1BGPBiliary glycoprotein 1
• BGPI
• Biliary Glycoprotein 1
• biliary glycoprotein adhesion molecule
• carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein)
• carcinoembryonic antigen-related cell adhesion molecule 1
• CD66a antigen
• CD66a
• Cea-1
• CEACAM1
• CEACAM-1
• Hv-1
• Hv-2
• MHVR

Background

CEACAM1 encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of only two has been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEACAM1