CDK8 Antibody - BSA Free

Immunoprecipitation:CDK8 AntibodyNBP3-29908] -Detection of human CDK8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK8 antibody NBP3-29908 (lot NBP3-29908-2) used for IP at 6 µg per reaction. CDK8 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29908-1). For blotting immunoprecipitated CDK8, NBP3-29908 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CDK8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CDK8 Antibody - BSA Free (NBP3-29908) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: between 370 and 420
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for CDK8 Antibody - BSA Free

• CDK8 protein kinase
• CDK8
• Cell division protein kinase 8
• cyclin-dependent kinase 8
• EC 2.7.11
• EC 2.7.11.22
• EC 2.7.11.23
• K35
• Mediator complex subunit CDK8
• Mediator of RNA polymerase II transcription subunit CDK8
• MGC126074
• MGC126075
• Protein kinase K35

Background

Cdk8 is encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK8