Cdk7 Overexpression Lysate (Native)

Images

 
Western Blot: Cdk7 Overexpression Lysate (Adult Normal) [NBL1-09045] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdk7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Cdk7 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202736 and based on accession number NM_001799. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cyclin-dependent kinase 7 (CDK7), mRNA.
Gene
CDK7

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Cdk7

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cdk7 Overexpression Lysate (Native)

  • CAK1Xenopus laevis, cdk-activating kinase)
  • CDK-activating kinase
  • Cell division protein kinase 7
  • cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase)
  • cyclin-dependent kinase 7
  • EC 2.7.11
  • EC 2.7.11.22
  • EC 2.7.11.23
  • EC 2.7.11.24
  • p39 Mo15
  • serine/threonine kinase stk1,39 kDa protein kinase
  • serine/threonine protein kinase 1
  • serine/threonine protein kinase MO15
  • TFIIH basal transcription factor complex kinase subunit

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Cdk7 Lysate (NBL1-09045) (0)

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Bioinformatics

Gene Symbol CDK7