CDK5RAP2 Antibody (CL3392) - Azide and BSA Free

Staining of HeLa cells using the anti-CDK5RAP2 monoclonal antibody) .

Staining of human breast cancer shows centrosome-like immunoreactivity in tumor cells.

Staining of A431 cells using the anti-CDK5RAP2 monoclonal antibody) .

Staining of human kidney shows centrosome-like positivity in renal cells.

Staining of U251 cells using the anti-CDK5RAP2 monoclonal antibody) .

Staining of human testis shows centrosome-like immunoreactivity in a subset of seminiferous epithelium cells.

Staining of human tonsil shows centrosome-like positivity in lymphoid cells.

Staining of U2OS cells using the anti-CDK5RAP2 monoclonal antibody) .

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL3392
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CDK5RAP2 Antibody (CL3392) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q96SN8, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDK5RAP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for CDK5RAP2 Antibody (CL3392) - Azide and BSA Free

• C48
• CDK5 activator-binding protein C48
• CDK5 regulatory subunit associated protein 2
• CDK5 regulatory subunit-associated protein 2
• centrosomal protein 215 kDa
• Centrosome-associated protein 215
• Cep215
• DKFZp686B1070
• DKFZp686D1070
• FLJ10867
• KIAA1633microcephaly, primary autosomal recessive 3
• MCPH3

Background

CDK5RAP2 is a potential regulator of CDK5 activity via its interaction with CDK5R1. This novel protein is widely expressed in human tissues, including the heart, brain, skeletal muscle, placenta, lung, liver, kidney and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK5RAP2