CDK5 Activator 1 Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

CDK5 Activator 1 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human CDK5 Activator 1
  Gln100-Arg307
  Accession # Q15078
• Specificity: Detects human CDK5 Activator 1 in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDK5 Activator 1 Antibody [Alexa Fluor® 700]

• CDK5 Activator 1
• CDK5P35
• CDK5R
• CDK5R1
• cyclin-dependent kinase 5 activator 1
• Cyclin-dependent kinase 5 regulatory subunit 1
• cyclin-dependent kinase 5, regulatory subunit 1 (p35)
• MGC33831
• NCK5A
• Neuronal CDK5 Activator
• p23
• p25
• p35
• p35nck5a
• regulatory partner for CDK5 kinase
• tau protein kinase II 23kDa subunit
• TPKII Regulatory Subunit

Background

Cyclin dependent kinase 5 regulatory subunit 1 (CDK5R1) is a 35 kDa protein that associates with and activates CDK5. Its expression is restricted to neurons and is required for normal brain development. CDK5R1 can be cleaved by calpain at Phe98-Ala99 which liberates a C-terminal fragment known as p25. p25 induces prolonged activation of CDK5 and broadens the substrate specificity of CDK5 to include tau which is aberrantly phosphorylated in Alzheimer's disease. Full length CDK5R1 is myristoylated at Gly2 and is preferentially localized to membranes at the cell periphery. p25 lacks this modification and is enriched in the nucleus and perinuclear regions. p25 accumulates in the brains of Alzheimer's patients where it promotes neuronal apoptosis and cytoskeletal abnormalities. Within aa 100-307, human CDK5R1 shares 99% aa sequence identity with mouse and rat CDK5R1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.