CDCP1 Antibody [Unconjugated]

CDCP1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4515) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing mouse brain. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CDCP1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CDCP1
  Arg25-Ala667
  Accession # Q5U462
• Specificity: Detects mouse CDCP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human CDCP1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CDCP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDCP1 Antibody [Unconjugated]

• CD318 antigen
• CD318
• CDCP1
• CUB domain containing protein 1
• CUB domain-containing protein 1
• Membrane glycoprotein gp140
• SIMA135
• SIMA135TRASK
• Subtractive immunization M plus HEp3-associated 135 kDa protein
• Transmembrane and associated with src kinases

Background

CDCP1 (CUB-domain containing protein 1; also CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aa’s 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350). Over aa’s 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDCP1 Antibody (AF4515)(4)

Publications using AF4515

• Liu, K;Yan, Z;Bai, D;Jiang, R;Bi, Y;Ma, X;Xiang, J;Sheng, Y;Dong, B;Ning, Z;Yi, S;Liu, Y;Lei, X;Jia, Y;Zhang, Y;Zhang, Y;Li, Y;Wu, T;Xi, C;Liu, S;Liu, S;Chen, J;Yin, J;Kou, X;Zhao, Y;Wang, H;Wang, Y;Wei, K;Gao, S;Liu, W; Modeling post-gastrula development via bidirectional pluripotent stem cells Cell research 2025-08-29 [PMID: 40883608] (Flow Cytometry, Mouse)
• Yiding Zhao, Qing Wang, Wenhao Zhang, Qingshen Jia, Qian Gao, Ling Shuai Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice STAR Protocols 2023-03-01 [PMID: 36853659]
• Mei Xu, Wenhao Zhang, Mengyang Geng, Yiding Zhao, Shengyi Sun, Qian Gao et al. Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system STAR Protocols 2021-12-01 [PMID: 34806042]
• A Yachie-Kin, K Onishi, J Ostblom, MA Langley, E Posfai, J Rossant, PW Zandstra Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions Mol. Syst. Biol., 2018-01-29;14(1):e7952. 2018-01-29 [PMID: 29378814] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CDCP1
• Uniprot:
  • Q5U462()