CDCP1 Antibody [Alexa Fluor® 594]

• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

CDCP1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CDCP1
  Arg25-Ala667
  Accession # Q5U462
• Specificity: Detects mouse CDCP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human CDCP1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CDCP1 (CUB-domain containing protein 1; also CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aa’s 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350). Over aa’s 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.