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CDC73/HRPT2 Antibody

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDC73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for CDC73 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF5508-SP
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CDC73/HRPT2 Antibody Summary

Immunogen
E. coli-derived recombinant human CDC73
Met1-Glu260
Accession # Q6P1J9
Specificity
Detects human and mouse CDC73 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDC73
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDC73/HRPT2 Antibody

  • C1orf28hyperparathyroidism 2 (with jaw tumor)
  • CDC73
  • cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S.cerevisiae)
  • Cell division cycle protein 73 homolog
  • chromosome 1 open reading frame 28
  • HPTJT
  • HPT-JT
  • HRPT2
  • HRPT2FLJ23316
  • Hyperparathyroidism 2 protein
  • HYX
  • Parafibromin

Background

CDC73 (also Parafibromin, HRPT2, and C1orf28) is a 60‑65 kDa member of the Cell Division Cycle family of proteins. It is found in adrenal, parathyroid and kidney, and serves as a regulator of Wnt signaling and a tumor suppressor by blocking c-myc expression. Human CDC73 is 531 amino acids (aa) in length. It contains multiple NLSs (aa 125‑139, 76‑92, and 393‑410) and a beta -catenin interaction sequence (aa 218‑263). There are at least two potential splice variants. One shows a five Lys substitution for aa 300‑531, while a second shows a 19 aa substitution for aa 298‑531. Full-length human and mouse CDC73 are completely (100%) identical in primary aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDC73
Uniprot